biosafety risk examples

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Appropriate respirators are chosen based on the type of work being performed. Five weeks later her illness was identified as an infection of Brucella, caused by a clinical sample processed in the lab where she worked without proper precautions (Noviello, et al., 2004). 5. A few items are provided in Microsoft Excel format. Biosafety Level 1: Suitable for work involving well-characterized agents not known to consistently cause disease in immunocompetent adult humans, and present minimal potential hazard to laboratory personnel and the environment. Although biosafety & biosecurity awareness and expertise has improved greatly in the past few decades through the availability more effective safety equipment and automated diagnostic technologies it remains one of the weakest core . and has providedtesting for those who would like to, or require testing. Biosafety Level 1. piece of chocolate. I am a very active person. The BSL categories are divided up by risk of disease combined with availability of preventive and therapeutic treatments. All persons entering the laboratory must be advised of the potential hazards and meet specific entry/exit requirements. He is wearing a full-body, air-supplied, positive pressure protective suit. For example, the non-pathogenic strain of E.coli is worked at the Biosafety level 1. What biosafety and biosecurity means to you and how it has affected your life. day. and containment; through the lab design and access restrictions; via personnel expertise;throughthe You know that your instructor is not going Ebola virus, Marburg virus, Sabia virus, Equine Morbillivirus. Equipment worn to minimize exposure to a variety of hazards. You assessed the hazardous characteristics of the potentially infectious the virus? If you have a safety concern, you can submit a Safety Concern Form or contact us at 612-626-6002. makes me a tiger. Keep laboratory doors closed; only individuals who are involved with the work are allowed in the area. and facility requirements (bandages) to keep you and your peers safe. The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with BSL-2 agents. What protective equipment and practices would you use? This review provides an overview of regulatory frameworks for biosafety and biosecurity in containment around the globe, as well as points out overlap with other regulatory . using your expertise (in this case, previous first hand experience and knowledge passed Does not cause disease in healthy adults. The sign must include the name and phone number of the laboratory supervisor or other responsible personnel, and may include the name of the agent(s) in use. Plastic ware should be substituted for glassware whenever possible. Mycobacterium tuberculosis, West Nile Virus, Yellow Fever Virus, Rickettsia rickettsi. If you work in a lab that is designated a BSL-1, the microbes there are not known to consistently cause disease in healthy adults and present minimal potential hazard to laboratorians and the environment. Join us in the month of October to celebrate biosafety, biosecurity and those that For CoVID19 information you can check out the Center for Disease Control or the Louisiana Department of Health for more information. Pay attention to hand and mouth hygiene while working in the lab (e.g., do not put a pen in you mouth, or behind your ear). After I graduated I went to work for as a postdoc They are so less toxic that they do not even need to be isolated from the building. This image suggests a BSL-4 laboratory. This includes how we handle animals, plants, cell culture, bacteria, viruses, fungi, parasites and public health crisis such as CoVID-19. that can result in a person's exposure, the likelihood that such exposure will cause There are also some casualties that often befall on people who do not expect anything to happen at any given moment. Salmonella and Shigella are examples of biohazards that have which Risk Group as their starting point in the risk assessment process? Well-characterized agents not known to consistently cause disease in immunocompetent adult humans, and that present minimal potential hazard to laboratory personnel and the environment. One example of a microbe with a level 1 rating (BSL-1) is E. coli. Or you attend a small party with about 15 people. Potential exposures to chemical hazards can occur both during use and with poor storage. Standard microbiological practices are those practices that are common to all laboratories. end of time. The table below containsa list ofagents and their categories. When i graduated high school I came to LSU to pursue you walk, choosing to wear pants and by taking a different route to class. Special PPE and facility construction are not necessary. Search Tips (search will sort by Species/Viral Group and show only top 500 matches) You can search partial names using the asterisk (*) Example: pseud* You can use Boolean operators OR, AND anthracis AND bacillus present is going around hugging everyone and touching all the food and drink. activities, I'm also an avid reader and I'm starting to get back into drawing. Select the Next icon to begin. Principal Investigators (PIs) are responsible for adhering to the following: Note: Completing the above steps does not entitle personnel to work with BSL-2 agents. The laboratorian is working with a pathogenic strain of Staphylococcus, which poses moderate risk of infection. operating procedures. Definitions. Perform all procedures with a focus onminimizing the creation of splashes and/or aerosols. OK guys and gals, let's get about as real world as you can get right now and talk In the United States, the Centers for Disease Control and Prevention (CDC) have specified these levels. Examples Comments Action Required (Y/N) (specify action) Aerosol generating potential centrifuging, vortexing, homogenizing, flaming loops Wear gloves, decontaminate work surfaces, avoid touching your face, make sure wounds are covered, work in a BSC, wear eye protection, work behind a shield. Report ADA Accessibility Concerns Incidents. a role in your risk of exposure. There are four biosafety levels. Biosafety Risk Assessment Standard Operating Procedure (SOP) and related documents, worksheets, tools are listed below. Before beginning work at BSL-1 researchers must, Before beginning work at BSL-2 researchers must. body does it affect? The objective is to estimate the nature and extent of the risks of a given situation (a priori analysis). Ladies and Gentlemen, that is risk, risk assessment and risk mitigation in a nutshell Before initial BSL-3 work, seek IBC approval prior to: For ongoing BSL-3 work seek IBC approval: 2022 By The Rector And Visitors Of The University Of Virginia, IBC - Institutional Biosafety Committee - Meetings & Members, IBC - Registration & Approval Process - PI Requirements, IBC - Inventory & Activity Registration (IAR), AAV - Adeno-Associated Viral Vectors - Policy for Biosafety Level Assignment, Human Gene Transfer (HGT) & Clinical Research, Medical Surveillance and Occupational Health, Recombinant or Synthetic Nucleic Acid Molecules, Risk Groups and working at BSL-1, BSL-2, or BSL-3, Transporting Infectious Substances by Ground, Food & Drink in Research Labs (UVA Policy), LABS - MOVING IN OR EXPANDING, ESTABLISHED LABS, Waste pickup & new container request - online request form, Federal Laser Product Performance Standard (FLPPS), RDRC - Radioactive Drug Research Committee, Dosimeter Application - online request form, How to Become Authorized to Use Radioactive Material, Radiation and Your Patient: A Guide for Medical Practitioners, Radiation Producing Equipment (Human Use & Non-Human Use), Radiation Survey Instruments (Calibration), Virginia Radiation Protection Regulations (12VAC5-481), Safety Program for Shops, Studios & Makerspaces, Hospital (generated in patient care areas), How to properly collect and dispose of your waste (all waste), ONLINE request form FOR WASTE REMOVAL (PICk-UP), STAR (Safety Training & Record-keeping) log-in, CDC-NIH Guidelines Biosafety in Microbiological and Biomedical Laboratories (BMBL), see UVA's Recombinant or Synthetic Nucleic Acid Molecules page for more information, IBC Inventory and Activity Registration (IAR) (log-in), Bloodborne Pathogen and Biosafety Training for Research Personnel (log-in), Bloodborne Pathogens and Biosafety Training for Research Personnel (log-in), Availability of effective preventive measures (e.g., vaccines), Availability of effective treatment (e.g., antibiotics), Human blood, blood components, fluids, unfixed organs, tissues and cell lines (primary and established), Non-Human Primate Derived Materials (including established cell lines), Biotoxins (with and LD 50 of less than 100 micrograms per kilogram of body weight in vertebrates) requiring BSL-2 containment, Research Involving Recombinant or Synthetic Nucleic Acid Molecules as described by the. Agent Type: Biohazard. Now that you have learned about the four biosafety levels, it's your turn to identify the correct BSL in the examples on the following screens. I was born and raised in Texas. Work can be performed on an open lab bench or table. Never eat, drink or smoke in the laboratory. BSL-2 builds upon BSL-1. Historically, the terms biosafety and biosecurity have had closely related and sometimes overlapping definitions. Every single person requires the basic necessitiesto survive--food, Commission Decision 2000/608/EC of 27 September 2000 concerning the guidance notes for risk assessment outlined in Annex III of Directive 90/219/EEC on the contained use of genetically modified micro-organisms - from Council . Report a Safety Concern. All work with microbes must be performed within an appropriate BSC. Member States to develop capacities to identify, store and securely handle dangerous biological agents and toxins according to international best practices. The descriptions of biosafety levels (BSL) 1 4 parallel those in the NIH Guidelines for research involving recombinant DNA. Two examples of microbes worked with in a BSL-4 laboratory include Ebola and Marburg viruses. Percutaneous, though broken or damaged skin, Mucous membranes of the eyes, nose, and mouth, Not eating, drinking, or applying cosmetics in the lab, Washing hands after working with infectious materials and before leaving the lab. Biosafety and biosecurity professionals have played a critical role inassuaging the Each biosafety level has its own specific containment controls that are required for the following: Route of exposure is the way a microbe gains access to a living organism. Biohazard Waste Examples. Agent Summary Statements are included for agents that meet one or more of the following three criteria: 1) the agent is a proven hazard to laboratory personnel working with infectious materials; 2) the agent has a high potential for causing LAIs even though no documented cases exist; and 3) the agent causes grave disease or presents a significant public health hazard. Biosafety Levels (BSLs) prescribe procedures and levels of containment for the particular microorganism or material (including research involving recombinant or synthetic nucleic acid molecules). Non-Discrimination Notice Minimal PPE, such as a lab coat, gloves, and eye protection might be worn but are not necessary. NOTE about Human Derived Materials (#2 above): Human or clinical materials needing IRB approval must be sought before IBC approval. grades were decent and I was able to get into my first choice college. For more information on biosafety and laboratory practice, review the following web resources: For more Quick-Learn lessons please visit CDC Learning Connection. Work surfaces after completion of work and after any spill or splash of potentially infectious material with appropriate disinfectant. 5 Potential Risks: Biosafety and Biosecurity. pathogenic or infections organisms) that pose a moderate health hazard. Step 1- Hazard Identification (What is this virus about?). You also decided that it would bea good idea to wear pants and a take a different Visitors and temporary personnel (i.e. EHS Biosafety follows the NIH Guidelines categorization of Risk Groups as follows: RG1 - Are not associated with disease in healthy adult humans or animals RG2 - Are associated with disease which is rarely serious and for which preventative or therapeutics is often available Work is conducted on a standard laboratory bench within a contained area, and the laboratorian is wearing appropriate PPE to reduce the risk of accidental infection. In both our professional and personal lives we have been asked to make significant When you are trying to identify what hazards are associated with the virus, the first Biological agents that are known to infect humans are classified according to Risk Groups (RG), with RG1 asthelowest/least harmful and RG4 asthe highest. Antonio, Texas and offered to take me with him as his lab manager. For example, the 2013 Paris Bettencourt team studied tuberculosis. Wash hands after all laboratory activities, following the removal of gloves, and immediately following contact with infectious agents. is based on recognition that hazards have the potential to be deliberately or accidentallymisused an outdoor gathering and everyone is maintaining their distance, but one of the folks The choices that you make and ultimately, the choices that othersmake will also play A sign incorporating the universal biohazard symbol must be posted at the entrance to the laboratory when infectious agents are present. To learn more about privacy at LSU, please see the LSU Privacy Statement. We are grateful for the impact your gifts make possible on the Hill. nearest bathroom to clean yourself up. Patrick Ceas Laboratory personnel have specific training in handling pathogenic agents and are supervised by scientists competent in handling infectious agents and associated procedures. Procedural hazards are going to be ever changing based on your daily routine and the Examples of PPE include laboratory coats, gowns, gloves, eye protection, face shields, shoe covers, and respirators. A hands-free sink and eyewash are available near the exit. Chemical Hygiene Officer Biosafety is the discipline that addresses the safe handling and containment of infectious Guess what? But I'm also loyal and honest with makes me like a lion. you mitigated the risk of acquiring an infection by cleaning the wound and you reduced The biosafety levels range from BSL-1 to BSL-4. This risk assessment SOP provides information . Biosafety in Microbiological and Biomedical Laboratories (5th Edition), Biosafety Centers for Disease Control and Prevention, U.S. Department of Health and Human Services. If you work in a lab that is designated a BSL-2, the microbes there pose moderate hazards to laboratorians and the environment. That is correct! The lab should have doors to separate the working space with the rest of the facility. Select the biosafety level described by the conditions of the following example. Additionalinformation Broken glassware must not be handled directly. Decontaminate work surfaces before and after use, and immediately after spills. Dispose of used gloves with other contaminated laboratory waste. Step 2- Procedural Hazards (What am I doing that has the potential to expose me to BSL-3 builds upon the containment requirements of BSL-2. Lab risks come in 3 forms: 1. the pandemic. It has become increasingly Source: National Institutes of Health Centers for Disease Control and Preventions , Autoclaves, Ice Machines, Liquid Nitrogen, Ultralows, Laboratory Safety: Guidelines & Rules for All Users, Preparing Chemical Solutions, Reagents, and Buffers, Standard Operating Procedures for Different Hazard Classes, Chlorine Bleach Safety (Incompatible Chemicals), Biological Exposure or Spills: Reponse, Decontamination & Clean-up, How to Determine if Your Waste is Hazardous, How to Segregate Your Containers of Hazardous Waste, Definition and Characterization of Hazardous Waste, Biosafety in Microbiological and Biomedical Laboratories. physical barriers were associated with each of the biosafety levels, and risk assess- I play on a women's and co-ed soccer team with the Baton BSLs are rankedfrom 1-4, with BSL-1 procedures being suitable for working withthelowest/least harmful biological agents. plants, cell culture, bacteria, viruses, fungi, parasites and public health crisissuch Use lab coats, gloves, safety eyewear, and other personal protective equipment. Lab personnel are handling specimens with little risk of infecting healthy human beings. Respiratory transmission is the inhalation route of exposure. Laboratory Supervisorsshould also conduct independent risk assessments before beginning work with an agent or procedure new to the laboratory, even though an agent summary statement is available. scale, the foundation isthe same. A needlestick injury is an example of direct . Stanford University follows the categorizing of infectious agents into levels as described inBiosafety in Microbiological and Biomedical Laboratories (BMBL), 5th edition, written and published by the Centers for Disease Control (CDC) and NIH. Guidelines for risk assessment, Biosafety Manuals. However, to maintain a similar observation scale with the biosafety element of NI, which was based on retrospective objective data, we did not further classify MSA or MTM risk events. resulting in harm. not really focused on anything but your upcoming weekend plans. changes to mitigate the risks associated with this pandemic. Most items are available to be opened or downloaded in the Portable Document Format (.PDF). Remove gloves and wash hands when work with hazardous materials has been completed and before leaving the laboratory. Are biosafety levels chosen based on risk assessments for every assay performed in your Highly specialized, totally encapsulating industrial protection garments worn only within special biocontainment or maximum containment (BSL-4) laboratory facilities. It Examples of changes that should include a new risk assessment include, but are not limited to, work with new organisms, different or new procedures, new or inexperienced . Rouge Soccer Club. Examples of agents typically worked with in a BSL-2 include equine encephalitis viruses and HIV, as well as Staphylococcus aureus (staph infections). Copyright Complaints, Biosafety & Biosecurity,Hazardous Materials,Lab Safety, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th edition, Animal Research Occupational Health and Safety Program, Hepatitis B Virus, Hepatitis C Virus, Hepatitis D Virus, Transmissible Spongiform Encephalopathies (Creutzfeldt-Jakob, kuru, and related agents. Laboratory supervisors should adopt improved engineering and work practice controls that reduce risk of sharps injuries. It was then that I grew to love this city and Ingestion, through the skin, and via facial mucous membranes. There are a small number of BSL-4 labs in the United States and around the world. Access to the laboratory is restricted when work is being conducted. A nonpathogenic strain of E. coli is being used, and work is conducted on a standard laboratory bench. While handling BSL-1 specimens, the lab doesn't need any advanced containment equipment. These experiments were a proof of principle for their project ideas. You get up, not thinking of a known infectious or potentially infectious agent or material, the activities At this point you head off to the Step 3- Personnel Hazards (What choicesam Imaking that have the potential to impact Since the beginning of 2020, the CoVID-19 pandemic has drastically altered (1) BIOSAFETY 1 is suitable for work involving well-characterized agents not known to cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment. In many countries, including the United States, biological agents are categorized in Risk Groups (RG) based on their relative risk. View Biosafety Risk Assessment-practical example (Vaccine production against Hepatitis B virus using a ba from CHEMICAL CH4223 at University of Moratuwa. I am fierce, confident and passionatewhich For example, they classified risk into internal and external risk, or management and practice risk. Depending on the country or organization, this classification system might take the following factors into consideration: It is important to understand that biological agents are classified in a graded fashion such that the level of hazard associated with RG1 being the lowest and RG4 being the highest. Work is conducted on a standard laboratory bench in a contained area. The list can be found by contacting EHS Biosafety. clear that tacking the pandemic and reducing the spread requires teamwork and cooperation. As a part of the CoVID19 prevention protocol, LSU has implemented Let's pretend that you are on campus headed to your first class of the terms: In the laboratory setting, risk is the probability that you, as the lab worker, will Biosafety is the application of safety precautions that reduce a laboratorians risk of exposure to a potentially infectious microbe and limit contamination of the work environment and, ultimately, the community. efforts. Hazards Associated with the Recipient/Host Susceptibility of the . Risk Group 4 agents require significant containment. read and sign the lab manual, and have the opportunity to ask questions. A known risk will lead to the implementation of appropriate prevention measures. Privacy Statement, Environmental Health and SafetyAdministrative Support BuildingLouisiana State UniversityBaton Rouge, LA 70803Telephone: 225-578-5640Fax: 225-578-7489. Ecological Workshop Risk Assessment irma.nps.gov Details File Format PDF Size: 2 MB Download 3. Infections caused by these microbes are frequently fatal and without treatment or vaccines. This example suggests a BSL-1 laboratory where there is minimal risk of infection. At the lowest level of the biosafety classification, level 1 is basically the least dangerous. A microbiology graduate student is working on a project under the following conditions: That is correct! KEY CONSIDERATIONS IN RISK ASSESSMENT. In some risk-prone circumstances, the Biosafety Level must be heightened for added protection from exposure/release of a biohazardous agent. or intentional misuse. identical. Wash hands after handling viable materials, after removing gloves, and before leaving the laboratory. What is Biosafety? I authorize that all information provided on this form, including any and all personal data may be shared with the LSU Office of Environmental Health and Safety and published anonymously on the Biological Safety and Biosafety webpages to facilitate community engagement through shared experiences. There are four biosafety levels. The common organisms that require Biosafety Level-1 containment include less hazardous organisms like Agrobacterium radiobacter, Aspergillus niger, Bacillus thuringiensis, Escherichia coli strain K12, Lactobacillus acidophilus, Micrococcus leuteus, Neurospora crassa, Pseudomonas fluorescens, Serratia marcescens. Under UCLA Policy 811, UCLA Policy 992 , and the Institutional Biosafety Plan, the following are some of the services we provide: Biosafety site assessments, reviews, consultations, small group discussions. The first level of Biosafety includes the least harmful agents. risk assessment is process used to identify and evaluate the hazardcharacteristics They correlate with but do not equate to biosafety risk groups. In high school, I was a wild child and always on the go. All work with the microbe must be performed within an appropriate. The laboratorian is working within a BSC and is wearing a powered air purifying respirator, gloves, and a solid-front gown. biohazards. If you work in a lab that is designated BSL-3, the microbes there can be either indigenous or exotic, and they can cause serious or potentially lethal disease through respiratory transmission. *A Note on the BMBL Agent Summary Statements Section VIIIof the BMBL provides Agent Summary Statements that describe the hazards, recommended precautions, and levels of containment appropriate for handling specific human and zoonotic pathogens in the laboratory and in facilities that house laboratory vertebrate animals. Common examples of agents found in a BSL-2 lab include equine encephalitis viruses, HIV, and staphylococcus aureus (staph infections). CDC 24/7: Saving Lives. This photograph suggests a BSL-3 laboratory. The correct answer is BSL-2. That is correct! Hepatitis B virus, HIV, the salmonellae, and Toxoplasma spp. Biosafety level one is the lowest level of precautions. What are Biosafety Levels (BSLs)? employed in research facilities to protect microbial agents from loss, theft, diversion I discovered that I really liked the regulatory side of science. In addition to BSL-1 considerations, BSL-2 laboratories have the following containment requirements: Equipment used to decontaminate biological hazardous waste or to sterilize equipment and supplies by subjecting them to high pressure and saturated steam, A ventilated cabinet designed to provide personnel, product, and environmental protection when appropriate practices and procedures are followed, An apparatus used to physically wash the eyes if they are contaminated by infectious microbes, foreign materials, or other substances, A protective device commonly used to shield the wearer's face and eyes from hazards such as the splashing, spraying, or splattering of potentially harmful infectious materials. This Biosafety Level requires at least two-door separation between the area where biohazards are used and . Bloodborne Pathogens (HBV, HCV, Borrelia). Biosafety Program Environmental Health & Safety Agent Classification The principal hazardous characteristics of an agent are: its capability to infect and cause disease in a susceptible human or animal host, its virulence as measured by the severity of disease, and the availability of preventive measures and effective treatments for the disease. This photograph suggests a BSL-3 laboratory. Eventually I decided The correct answer is BSL-4. 3 months or less) must also complete the online training course, Before changing the scope or location of your existing experiments, Before providing BSL-3 agents to another investigator within or outside UVA, Before arranging for visiting researchers to work in your BSL-3 laboratory. NOTE: Risk Groups, as defined below, apply to healthy adults; immunocompromised individuals are more vulnerable to infectious disease and thus could be at greater risk if exposed. campus and the reason that I now call Baton Rouge home. to let you in class with a bloody leg and you want to avoid the possibility of an risk analysis methods are commonly divided into It is the PI or supervisors responsibility to assess an individuals readiness to work with BSL-2 agents, and also to directly demonstrate and train new personnel in the specific techniques and practices that are used in their lab. in mitigating emerging risk. Materials to be decontaminated outside of the immediate laboratory must be placed in a durable, leak proof container and secured for transport. Because of inhalation risk, perform all work inside of a biosafety cabinet. Parasites (i.e. You will learn about each level on the following screens. On this page Introduction The responsibilities microorganisms and hazardous biological materials. Biological research-related incident investigations and response. Capable of causing severe disease with lethal outcome. Risk Group 2. Medical Surveillance and additional training is required. Entrance to the lab is through two sets of self-closing and locking doors. You go in and This Quick Learn Lesson will take approximately 15 minutes to complete. Detailed descriptions of containment practices and biosafety levels can be found in the CDC-NIH Guidelines Biosafety in Microbiological and Biomedical Laboratories (BMBL). Each level has specific controls for containment of microbes and biological agents. Specific considerations for a BSL-1 laboratory include the following: A nonpathogenic microbe is one that is not capable of causing disease. The American Biological Safety Association (ABSA) presents October as Biosafety and You are then Shortly after I arrived at my new job, Dr. Kaushal informedme that he had taken the team outside of school. Wear pants (or other clothing that covers legs) and close-toed shoes. A That is correct! Work is conducted on a standard laboratory bench within a contained area, and the laboratorian is wearing appropriate PPE to reduce the risk of accidental infection. An example of a microbe that is typically worked with at a BSL-1 is a nonpathogenic strain of E. coli. The consideration of such cases should include an estimation of the level of expression required to achieve biological or pharmacological activity. Suddenly, you trip Every microbiology laboratory, regardless of biosafety level, follows standard microbiological practices. Plasmodium, Trypanosomes, Leishmania). To view other Quick Learn Lessons, visit CDC Learning Connection. Examples of agents that can be worked with at BSL-2 include hepatitis A virus, herpes simplex virus, Toxoplasma gondii, Staphylococcus aureus, Streptococcus pyogenes, Salmonella species and many other foodborne pathogens. this is achieved via the principles of containment and risk assessment. You have just performed a risk assessment of your situation without even Select the biosafety level shown in the following photograph below. AllRightsReserved. BSL-4 builds upon the containment requirements of BSL-3 and is the highest level of biological safety. Hand washing protocols must be rigorously followed. 7. Biosafety is the application of safety precautions that reduce a laboratorian's risk of exposure to a potentially infectious microbe and limit contamination of the work environment and, ultimately, the community. These cabinets are provided with HEPA-filters that decontaminate the air moving out of the cabinet. & Emergencies. Refer to the biosafety level and risk group definitions in the table below: BSL-1: Biosafety Level 1: Preventing the accidental release or contamination of research samples; and . The following week when you 1520 St. Olaf Avenue Biosafety is the prevention of large-scale loss of . the lady behind you didn't. Experimental treatment regimens possible. Appropriate personal protective equipment (PPE), All procedures that can cause infection from aerosols or splashes are performed within a. Laboratorians are under medical surveillance and might receive immunizations for microbes they work with. BSL-1 practices are used for work with agents that pose a minimal risk to workers or the environment and do not typically cause disease in healthy adults. Rarely lethal. What are Biosafety Levels (BSLs)? For example, a specific research project's biological risk assessment for the use of human immunodeficiency virus (HIV), a Risk Group 3 agent, may correctly determine that HIV can be handled under Biosafety Level 2 conditions. Each laboratory space where biohazardous materials are used is assigned one of 3 internationally recognized biosafety levels or BSL. Biosafety Levels (BSL) Research and teaching activities involving infectious agents requires prior approval by the Institutional Biosafety Committee (IBC) via the Biohazard Use Authorization (BUA) review process. Examples of biosafety considerations and risk assessments for three different scenarios are included below. 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